Organoids are complex three-dimensional in vitro organ-like model systems. Organoids can be derived from pluripotent stem cells or primary donor tissue and have been used to address fundamental questions about development, stem cell biology and organ regeneration. Human organoids have invigorated new exploration into the cellular makeup of human organs during development, in the adult and during disease. Efforts to improve complexity in organoid systems, and to make organoid systems more robust, reproducible and controlled have prompted continued refinement of methods used to generate and culture organoids. This volume will highlight recent and emerging advances that describe organoid differentiation protocols for many different organ systems, that implement organoids as tools to understand complexity and maturation, high content drug screening, disease modeling, and understanding development and evolution. Readers can expect to gain knowledge in the most recent differentiation protocols from experts in their respective areas.
Organoids are complex three-dimensional in vitro organ-like model systems. Organoids can be derived from pluripotent stem cells or primary donor tissue and have been used to address fundamental questions about development, stem cell biology and organ regeneration. Human organoids have invigorated new exploration into the cellular makeup of human organs during development, in the adult and during disease. Efforts to improve complexity in organoid systems, and to make organoid systems more robust, reproducible and controlled have prompted continued refinement of methods used to generate and culture organoids. This volume will highlight recent and emerging advances that describe organoid differentiation protocols for many different organ systems, that implement organoids as tools to understand complexity and maturation, high content drug screening, disease modeling, and understanding development and evolution. Readers can expect to gain knowledge in the most recent differentiation protocols from experts in their respective areas.
1. Pluripotent stem cell derived gastric organoids
Yana Zavros
2. Pluripotent stem cell derived esophageal organoids
James Wells
3. Pluripotent stem cell derived small intestinal organoids
David R. Hill
4. Pluripotent stem cell derived colonic organoids
Jorge Munera
5. Pluripotent stem cell intestinal organoids with an Enteric
Nervous System
Maxime Mahe
6. Pluripotent stem cell derived airway organoids
Finn Hawkins
7. Pluripotent stem cell derived alveolar organoids
Shimpei Gotoh
8. Pluripotent stem cell derived liver bud organoids
Takanori Takebe
9. Pluripotent stem cell derived thyroid organoids
Sabine Costagliola
10. Pluripotent stem cell derived renal organoids
Jennifer Harder
11. Pluripotent stem cell derived cerebral organoids
Gray Camp
12. Generating ventral spinal organoids from human induced
pluripotent stem cells
Shi Yan Ng
13. Pluripotent stem cell derived retinal organoids
Jason Meyer
14. Pluripotent stem cell derived inner ear organoids
Eri Hashino
15. Pluripotent stem cell derived skin
Karl R. Koehler
16. Pluripotent stem cell derived cholangiocytes and cholangiocyte
Organoids
Anne Dubart-Kupperschmitt
Jason R. Spence, PhD, is an Associate Professor of Internal
Medicine, Cell and Developmental Biology and Biomedical Engineering
at the University of Michigan Medical School. He attended Canisius
College in Buffalo, NY, as an undergraduate. He attended graduate
school at Miami University (Ohio) where his research focused on
understanding mechanisms that drive regeneration and tissue repair
in unique model organisms that maintain regenerative ability
throughout life, including Notophthalmus viridescens (Eastern
Newt), Ambystoma mexicanum (Axolotl) and the chick. He performed
postdoctoral research Cincinnati Children’s Hospital, where he
turned his focus to understanding mechanisms that regulate
embryonic development of endoderm-derived tissue (pancreas, liver,
intestine) and utilized human pluripotent stem cells (hPSCs) to
understand human differentiation and development. During this time,
he pioneered methods to differentiate 3-dimensional intestinal
organoids from human pluripotent stem cells.
In 2011, Dr. Spence joined the faculty of the University of
Michigan Medical School. The focus of the Spence lab include using
3-dimensional organoid human models to study human development and
disease, with research focused on understanding intestinal, lung
and esophageal development, homeostasis and disease.
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