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The Nobel Prize was awarded in Physiology or Medicine in 1998 to Louis J. Ignarro, Robert F. Furchgott and Ferid Murad for demonstrating the signaling properties of nitric oxide. Nitric Oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. NO research has led to new treatments for treating heart as well as lung diseases, shock and impotence. (Sildenafil, popularly known by the trade name Viagra, enhances signaling through nitric oxide pathways.) Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis. This is another "must-have" volume packed with robust methods from authors around the globe. Researchers interested in the detailed biochemistry of nitric oxide and its synthesis will have this indispensable volume on their shelves. *Essential resource for every laboratory involved in NO-related work *Gathers tried and tested techniques from global labs which eliminates searching through many different sources and avoids pitfalls so the same mistakes are not made over and over.* Aids researchers in the design of medically important therapies for heart disease and cancer
The Nobel Prize was awarded in Physiology or Medicine in 1998 to Louis J. Ignarro, Robert F. Furchgott and Ferid Murad for demonstrating the signaling properties of nitric oxide. Nitric Oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. NO research has led to new treatments for treating heart as well as lung diseases, shock and impotence. (Sildenafil, popularly known by the trade name Viagra, enhances signaling through nitric oxide pathways.) Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis. This is another "must-have" volume packed with robust methods from authors around the globe. Researchers interested in the detailed biochemistry of nitric oxide and its synthesis will have this indispensable volume on their shelves. *Essential resource for every laboratory involved in NO-related work *Gathers tried and tested techniques from global labs which eliminates searching through many different sources and avoids pitfalls so the same mistakes are not made over and over.* Aids researchers in the design of medically important therapies for heart disease and cancer
Cadenas and Packer continue the trend with their stellar collection of volumes in recruiting the world's best researchers to present robust, cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues.
Chapter One: Protein 3-Nitrotyrosine in Complex Biological Samples:
Quantification by High-Pressure Liquid
Chromatography/Electrochemical Detection and Emergence of Proteomic
Approaches for Unbiased Identification of Modification Sites
Chapter Two: Selective Fluorogenic Derivatization of
3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A
Method Designed for Quantitative Proteomic Analysis
Chapter Three: Nitroalkenes: Synthesis, Characterization, and
Effects on Macrophage Activation
Chapter Four: In-Gel Detection of S-Nitrosated Proteins Using
Fluorescence Methods
Chapter Five: The Arachidonate-Dependent Survival Signaling
Preventing Toxicity in Monocytes/Macrophages Exposed to
Peroxynitrite
Chapter Six: Practical Approaches to Investigate Redox Regulation
of Heat Shock Protein Expression and Intracellular Glutathione
Redox State
Chapter Seven: Monitoring Oxidative Stress in Vascular Endothelial
Cells in Response to Fluid Shear Stress: From Biochemical Analyses
to Micro- and Nanotechnologies
Chapter Eight: Determination of S-Nitrosothiols in Biological and
Clinical Samples Using Electron Paramagnetic Resonance Spectrometry
with Spin Trapping
Chapter Nine: Novel Method for Measuring S-Nitrosothiols Using
Hydrogen Sulfide
Chapter Ten: Kinetic Studies on Peroxynitrite Reduction by
Peroxiredoxins
Chapter Eleven: Nitrocytochrome c: Synthesis, Purification, and
Functional Studies
Chapter Twelve: Tyrosine Nitration, Dimerization, and Hydroxylation
by Peroxynitrite in Membranes as Studied by the Hydrophobic Probe
N-T-BOC-L-tyrosine tert-Butyl Ester
Chapter Thirteen: Assessment of Superoxide Production and NADPH
Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation
Products
Chapter Fourteen: Methods to Measure the Reactivity of
Peroxynitrite-Derived Oxidants Toward Reduced Fluoresceins and
Rhodamines
Chapter Fifteen: Detection and Characterization of
Peroxynitrite-Induced Modifications of Tyrosine, Tryptophan, and
Methionine Residues by Tandem Mass Spectrometry
Chapter Sixteen: Reductive Gas-Phase Chemiluminescence and Flow
Injection Analysis for Measurement of the Nitric Oxide Pool in
Biological Matrices
Chapter Seventeen: Detection and Measurement for the Modification
and Inactivation of Caspase by Nitrosative Stress In Vitro and In
Vivo
Chapter Eighteen: Interactive Relations between Nitric Oxide (NO)
and Carbon Monoxide (CO): Heme Oxygenase-1/CO Pathway Is a Key
Modulator in NO-Mediated Antiapoptosis and Anti-inflammation
Chapter Nineteen: Detection and Characterization of In Vivo
Nitration and Oxidation of Tryptophan Residues in Proteins
Chapter Twenty: In Vivo Real-Time Measurement of Nitric Oxide in
Anesthetized Rat Brain
Chapter Twenty-One: Nitric Oxide and Cardiobiology-Methods for
Intact Hearts and Isolated Myocytes
Chapter Twenty-Two: Microscopic Technique for the Detection of
Nitric Oxide-Dependent Angiogenesis in an Animal Model
Author Index
Subject Index
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.
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