There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research. It combines classical approaches and modern technologies. It presents easy-to-use protocols and thorough background information, compiled by leaders in the field.
There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research. It combines classical approaches and modern technologies. It presents easy-to-use protocols and thorough background information, compiled by leaders in the field.
2e of the Methods in Cell Biology volume on microtubules
1. Isolation of Antarctic fish tubulin and MAPs
2. Making, isolating and characterizing tubulin alpha
antibodies
3. Techniques for labeling tubulin and drugs
4. Single-particle 3D reconstruction methods for decorated and
undecorated microtubules
5. A new microtubule gliding assay and analysis of microtubule
persistence length
6. Characterization of gamma tubulin
7. Biochemical and Structural Studies of the doublecortin family of
MAPs
8. Detection and quantification of microtubule detachment from
centrosomes and spindle poles
9. Regulation of tubulin expression by micro RNAs: Implications for
Drug Resistance
10. A portable magnetic tweezers device to visualize
three-dimensional deformation of reconstituted microtubule
networks
11. Mitochondrial/microtubule interactions – mmbp1
12. Going Solo: Measuring the motions of microtubules with an in
vitro assay for TIRF microscopy
13. Analysis of Microtubules and Motors in Isolated Axoplasm from
the Squid Giant Axon
14. Analysis of drug binding to microtubules and steady state
microtubule growth/treadmilling by use of a sedimentation assay in
vitro
15. Specific recombinant antibody technology for the study of
microtubule dynamics and regulatory proteins
16. Tubulin-specific Chaperones: Components of a Molecular Machine
that Assembles the aB Heterodimer
17. G-protein tubulin interactions
18. Katanin, a microtubule severing enzyme: expression,
purification, and biophysical characterization
19. Measuring tubulin polymerization in vitro – comparison of
methods and possible artifacts
20. Imaging microtubules in living plant cells
21. How to use which antibody known in the field, what to expect
from this antibody and how semi-quantitative data can be reliably
obtained with antibody staining
22. Preparation of bacterial tubulin BtubA/B and constructs bearing
eukaryotic tubulin sequences
23. Microtubule-associated proteins and tubulin interaction by
isothermal titration calorimetry
24. TBC
25. Characterization of the mechanism of action of the
covalent-binding microtubule-stabilizing agent zampanolide
26. Studying kinetochore-fibre ultrastructure using correlative
light-electron microscopy
27. Fluorescence-based Assays for Microtubule Structure
28. Yeast Tubulin-structure-function analysis
29. Microtubule Binding SIm to simulate binding of proteins to
polymers under different binding models
University of California, Santa Barbara, USA
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