This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy.

Part I: PINK 1/Parkin-mediated Mitophagy

1. Short Overview of PINK1/Parkin-mediated Mitophagy

Norihiko Furuya

2. Induction of PINK1/Parkin-Mediated Mitophagy

Shigeto Sato And Norihiko Furuya

3. Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells

Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi, and Nobutaka Hattori

4. The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy

5. Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells

Atsushi Hoshino and Satoaki Matoba

6. Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila

Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Hongrui Meng, Nobutaka Hattori, and Yuzuru Imai

7. Assessment of Mitophagy in iPS Cell-derived Neurons

Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, and Wado Akamatsu

PART II: Mitophagy Associated with Nix

8. Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting

Sachiyo Nagumo And Koji Okamoto

9. Mitopho8¿60 Assay as a Tool to Quantitatively Measure Mitophagy Activity

Zhiyuan Yao, Xu Liu And Daniel J. Klionsky

10. Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants

Kentaro Furukawa and Tomotake Kanki

11. Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy

Matilda sprung, Ivan Dikic, and Ivana Novak

12. Exploring Micrornas on Nix-Dependent Mitophagy

Wen Li, Hao Chen, Shupeng Li, Guanghong Lin, and Du Feng

Part III: Others

13. Monitoring of Atg5-Independent Mitophagy

Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka, and Shigeomi Shimizu

14. Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans

Ken Sato and Miyuki Sato

15. Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells

Shun-Ichi Yamashita and Tomotake Kanki

16. Monitoring Mitophagy during Ageing in Caenorhabditis Elegans

Nikolaos Chrmpilas, Konstantinos Kounakis, and Nektarios Tavernarakis

17. Iron-Starvation Induced Mitophagy in Yeast

Koichi Tanabe