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Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.
1. Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering
Benjamin M. Buehrer and Bentley and Cheatham
2. Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs
Darell W. McCoy
3. Isolation of Urothelial Cells from Bladder Tissue
Namrata Sangha
4. Isolation of Pulsatile Cell Bodies from Esophageal Tissue
John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, and Namrata Sangha
5. Cell Isolation Through Whole Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis
Sonya O. Meheux
6. Ex Vivo Culture and Separation of Functional Renal Cells
Andy Bruce, Kelly Guthrie, and Rusty Kelley
7. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells (MSC) for Urologic Tissue Engineering
Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, and Yuanyuan Zhang
8. Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells
Tori Sampsell-Barron
9. Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes
Gary R. Skuse and Kathleen A. Lamkin-Kennard
10. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine
Teresa Bora Burnette and Andrew T. Bruce
11. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications
Monica A. Serban, Toyin A. Knight, and Richard G. Payne
12. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration
Adelola O. Oseni, Peter E. Butler, and Alexander M. Seifalian
13. Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features
Scott Rapoport
14. Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering
Richard G. Payne and Toyin A. Knight
15. Characterization of a PGA Based Scaffold for Use in a Tissue Engineered Neo-Urinary Conduit
Toyin A. Knight and Richard G. Payne
16. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications
Kelly Guthrie, Namrata Sangha, Christopher Genheimer, Joydeep Basu, and John W. Ludlow
17. Care of Rodent Models used for Pre-Clinical Evaluation of Tissue Engineered/Regenerative Medicine Product Candidates
Kim L. Mihalko
18. Bioreactor Design Considerations for Hollow Organs
Jeff Fish, Craig Halberstadt, Darell McCoy, and Neil Robbins
19. Construction of a Multicoaxial Hollow Fiber Bioreactor
Randall McClelland, Katherine Tech, and Jeffrey M. Macdonald
20. Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas
Emmanuel C. Opara, John P. McQuilling, and Alan C. Farney
21. Bioengineered Skin Substitutes
Pedro Lei, Hui You, and Stelios T. Andreadis
22. Formulation of Selected Renal Cells for Implantation into a Kidney
Craig Halberstadt, Neil Robbins, Darell McCoy, Kelly Guthrie, Andrew Bruce, Toyin Knight, and Richard Payne
23. Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold
Pedro M. Baptista, Dipen Vyas, Emma Moran, Zhan Wang, and Shay Soker
24. A "Living Bioreactor" for the Production of Tissue Engineered Small Intestine
Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torashima, and Tracy C. Grikscheit
25. Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models
Joydeep Basu, Kim Mihalko, Elias Rivera, Kelly I. Guthrie, Christopher Genheimer, Namrata Sangha, and John W. Ludlow
26. Scanning Electron Microscopy Evaluation of Endothelialized Tissue Engineered Constructs
Sandra L. Johnson
27. Genotypic and Phenotypic Analysis of in vivo Tissue Regeneration in an Animal Model
Christopher W. Genheimer
28. Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organs Applications
Elias A. Rivera and Manuel J. Jayo
Show moreTissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.
1. Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering
Benjamin M. Buehrer and Bentley and Cheatham
2. Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs
Darell W. McCoy
3. Isolation of Urothelial Cells from Bladder Tissue
Namrata Sangha
4. Isolation of Pulsatile Cell Bodies from Esophageal Tissue
John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, and Namrata Sangha
5. Cell Isolation Through Whole Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis
Sonya O. Meheux
6. Ex Vivo Culture and Separation of Functional Renal Cells
Andy Bruce, Kelly Guthrie, and Rusty Kelley
7. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells (MSC) for Urologic Tissue Engineering
Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, and Yuanyuan Zhang
8. Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells
Tori Sampsell-Barron
9. Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes
Gary R. Skuse and Kathleen A. Lamkin-Kennard
10. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine
Teresa Bora Burnette and Andrew T. Bruce
11. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications
Monica A. Serban, Toyin A. Knight, and Richard G. Payne
12. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration
Adelola O. Oseni, Peter E. Butler, and Alexander M. Seifalian
13. Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features
Scott Rapoport
14. Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering
Richard G. Payne and Toyin A. Knight
15. Characterization of a PGA Based Scaffold for Use in a Tissue Engineered Neo-Urinary Conduit
Toyin A. Knight and Richard G. Payne
16. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications
Kelly Guthrie, Namrata Sangha, Christopher Genheimer, Joydeep Basu, and John W. Ludlow
17. Care of Rodent Models used for Pre-Clinical Evaluation of Tissue Engineered/Regenerative Medicine Product Candidates
Kim L. Mihalko
18. Bioreactor Design Considerations for Hollow Organs
Jeff Fish, Craig Halberstadt, Darell McCoy, and Neil Robbins
19. Construction of a Multicoaxial Hollow Fiber Bioreactor
Randall McClelland, Katherine Tech, and Jeffrey M. Macdonald
20. Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas
Emmanuel C. Opara, John P. McQuilling, and Alan C. Farney
21. Bioengineered Skin Substitutes
Pedro Lei, Hui You, and Stelios T. Andreadis
22. Formulation of Selected Renal Cells for Implantation into a Kidney
Craig Halberstadt, Neil Robbins, Darell McCoy, Kelly Guthrie, Andrew Bruce, Toyin Knight, and Richard Payne
23. Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold
Pedro M. Baptista, Dipen Vyas, Emma Moran, Zhan Wang, and Shay Soker
24. A "Living Bioreactor" for the Production of Tissue Engineered Small Intestine
Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torashima, and Tracy C. Grikscheit
25. Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models
Joydeep Basu, Kim Mihalko, Elias Rivera, Kelly I. Guthrie, Christopher Genheimer, Namrata Sangha, and John W. Ludlow
26. Scanning Electron Microscopy Evaluation of Endothelialized Tissue Engineered Constructs
Sandra L. Johnson
27. Genotypic and Phenotypic Analysis of in vivo Tissue Regeneration in an Animal Model
Christopher W. Genheimer
28. Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organs Applications
Elias A. Rivera and Manuel J. Jayo
Show moreIsolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering.- Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs.- Isolation of Urothelial Cells from Bladder Tissue.- Isolation of Pulsatile Cell Bodies from Esophageal Tissue.- Cell Isolation Through Whole Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis.- Ex Vivo Culture and Separation of Functional Renal Cells.- Isolation and Myogenic Differentiation of Mesenchymal Stem Cells (MSC) for Urologic Tissue Engineering.- Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells.- Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes.- Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine.- Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications.- Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration.- Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features.- Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering.- Characterization of a PGA Based Scaffold for Use in a Tissue Engineered Neo-Urinary Conduit.- Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications.- Care of Rodent Models used for Pre-Clinical Evaluation of Tissue Engineered/Regenerative Medicine Product Candidates.- Bioreactor Design Considerations for Hollow Organs.- Construction of a Multicoaxial Hollow Fiber Bioreactor.- Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas.- Bioengineered Skin Substitutes.- Formulation of Selected Renal Cells for Implantation into a Kidney.- Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold.- A "Living Bioreactor" for the Production ofTissue Engineered Small Intestine.- Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models.- Scanning Electron Microscopy Evaluation of Endothelialized Tissue Engineered Constructs.- Genotypic and Phenotypic Analysis of in vivo Tissue Regeneration in an Animal Model.- Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organs Applications.
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