Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation. In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins such as B, NK, myeloid, and dendritic cells, as well as the role of several suppressive molecules in immunoregulation. Particular emphasis is placed on the characterization of the molecular mechanisms and the therapeutic applications of regulatory cells and molecules in human diseases. Written as a volume in the highly successful Methods in Molecular Biology(TM) series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.



Comprehensive and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols serves as a key reference for scientists seeking a way toward greater control over the enormous power of the complex and vital immune system.

Part I: Regulatory Cells 1. Natural and Induced T CD4+CD25+FOXP3+ Regulatory T Cells Lucienne Chatenoud 2. Polyclonal Expansion of Human CD4+CD25+ Regulatory T Cells Petra Hoffmann, Ruediger Eder, and Matthias Edinger 3. Methods for In vitro Generation of Human Type 1 Regulatory T (Tr1) Cells Silvia Gregori, Maria Grazia Roncarolo, and Rosa Bacchetta 4. Ex vivo Generation of Regulatory T-Cells: Characterization and Therapeutic Evaluation in a Model of Chronic Colitis Fridrik Karlsson, Sherry Jackson, Laura Gray, Songlin Zhang, and Matthew B. Grisham 5. Phenotypic and Functional Characterization of CD8+ T Regulatory Cells Séverine Ménoret, Carole Guillonneau, Séverine Bezié, Lise Caron, Ignacio Anegon, and Xian-Liang Li 6. Regulatory CD4- CD8- Double Negative T Cells Edward Y. Kim, Stephen C. Juvet, and Li Zhang 7. Identifying Regulatory B Cells (B10 Cells) that Produce IL-10 in Mice Takashi Matsushita and Thomas F. Tedder 8. DCs in Immune Tolerance in Steady-State Conditions Tomohiro Fukaya, Hideaki Takagi, Honami Taya, and Katsuaki Sato 9. Plasmacytoid Dendritic Cells in Tolerance Eric Gehrie, William Van der Touw, Jonathan S. Bromberg, and Jordi C. Ochando 10. In vitro Generated DC with Tolerogenic Functions: Perspectives for In vivo Cellular Therapy Cees van Kooten and Kyra A. Gelderman 11. Preparation of Mouse Bone Marrow-Derived Dendritic Cells with Immunoregulatory Properties Mercedes Segovia, Maria Cristina Cuturi, and Marcelo Hill 12. Myeloid Derived Suppressor Cells (MDSC): Characterization and Expansion in Models of Endotoxemia and Transplantation Nicolas Van Rompaey and Alain Le Moine 13. Human Regulatory Macrophages James A. Hutchinson, Paloma Riquelme, Edward K. Geissler, and Fred Fändrich 14. NKT and Tolerance Julien Diana, Lucie Beaudoin, Anne-Sophie Gautron, and Agnès Lehuen 15. Anti-Inflammatory and Immunosuppressive Functions of Mast Cells Janet Kalesnikoff and Stephen J. Galli 16. A Mesenchymal Stem Cell Potency Assay Joy Jiao, Jack M. Milwid, Martin L. Yarmush, and Biju Parekkadan 17. In Vitro Analyses of the Immunosuppressive Properties of Neural Stem/Progenitor Cells Using Anti-CD3/CD28 Activated T Cells Virginie Bonnamain, Isabelle Neveu, and Philippe Naveilhan Part II: Regulatory Molecules 18. Immunoregulatory Properties of Heme Oxygenase-1 Philippe Blancou, Virginie Tardif, Thomas Simon, Séverine Rémy, Leandro Carreño, Alexis Kalergis, and Ignacio Anegon 19. Indoleamine, 2,3-Dioxygenase, and Regulatory Function: Tryptophan Starvation and Beyond Ciriana Orabona and Ursula Grohmann 20. Regulatory T Cell Enrichment by IFN-¿ Conditioning Gang Feng, Kathryn J. Wood, and Andrew Bushell 21. Transforming Growth Factor-Beta: Recent Advances on Its Role in Immune Tolerance Pierre-Yves Mantel and Carsten B. Schmidt-Weber 22. Induction of Tolerogenic Dendritic Cells by NF-¿B Blockade and Fc¿ Receptor Modulation Leandro J. Carreño, Claudia A. Riedel, and Alexis M. Kalergis 23. Fine-Tuning Antitumor Responses through the Control of Galectin-Glycan Interactions: An Overview Mariana Salatino and Gabriel A. Rabinovich 24. Regulation of Lymphocytes by Nitric Oxide Christian Bogdan Part III: Physiopathological Situations 25. Pregnancy: Tolerance and Suppression of Immune Responses Ana Claudia Zenclussen, Anne Schumacher, Maria Laura Zenclussen, Ana Teles, Nadja Brachwitz, Pablo Casalis, Tarek El-Mousleh, Federico Jensen, and Katja Woidacki 26. Organ Transplantation: Modulation of T Cell Activation Pathways Initiated by Cell Surface Receptors to Suppress Graft Rejection Kathleen Weatherly and Michel Y. Braun 27. Immunosuppressive Mechanisms During Viral Infectious Diseases Ghanashyam Sarikonda and Matthias G. von Herrath 28. Ocular Immune Privilege Sites Sharmila Masli and Jose L. Vega 29. Immunoprivileged Sites: The Testis Monika Fijak, Sudhanshu Bhushan, and Andreas Meinhardt